DNA methylation is an important epigenetic mark information, and obtaining the methylation level data of all C sites in the whole genome is of great significance for the spatiotemporal specific study of epigenetics. Bisulfite methylation sequencing is a new generation of high-throughput sequencing platform based on a combination of genome-wide Bisulfite processing and bioinformatics data analysis technology to map genome-wide DNA methylation levels.
| Product Type | Sequencing Strategy | Recommended Data Amount | Cycle |
| WGBS | NovaSeq 6000 PE150 DNBSEQ-T7 PE150 | Average sequencing depth ＞30X | 60days (standard analysis) |
| Product Type | Sampling Requirements |
| Whole Genome Methylation | Sample type: DNA samples free of degradation and RNA contamination |
| Sample demand: ≥ 0.5μg | |
| Sample concentration: ≥13 ng/μL | |
| OD260/280=1.8-2.2 | |
| Low starting quantity situation: a total DNA amount of ≥ 50 ng; A concentration of ≥ 0.2 ng/μl; No protein and RNA contamination. |